Apoptotic effects of Phenolic extract from Spirulina platensis on Esophagus cancer cell line SK-GT-4

Abstract

Author(s): Rasha. N. Aljabery*, Mohammed A. Auda and Hussain Yosif Al-Rekabi

Due to their strong antioxidant qualities and their notable impact in the prevention of numerous oxidative stress-related disorders like cancer, phenolic extract from Spirulina platensis has attracted increasing attention. Phenolic compounds were extracted from spirulina platensis by using an ultrasonic-assisted extraction method. The yield of Phenolic extract by this method was (0.21% w/w). Additionally, phenolic components for phenolic extract were identified using the HPLC technique. The findings indicated that the extract contained a number of important compounds, including vanillic acid, gallic acid, Ferulic acid, cinnamic acid. Then, using a fluorescent microscope, the Acridine-orange-Ethidium bromide (AaO/ EtBr) double labelling was utilized to identify the alterations in nuclear morphology during apoptosis. SK-GT-4 cells were exposed to phenolic extract for 72 hours. The findings demonstrated that treatment with the extract causes somewhat more apoptosis in treated cells than in untreated cells.

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