Clinicopathological features' correlation and genes? expression of NEAT1, lincRNAROR and Oct4 in Iranian patients with gastric cancer

Abstract

Author(s): Vahid Chaleshi, Mahyar Nourian, Naghmeh Zamani, Narjes Mehrvar, Shahrokh Iravani, Hasan Jalaeikhoo, Hamid Asadzadeh Aghdaei

Purpose: Long non-coding RNAs (LncRNAs) play a critical role in the initiation and development of Gastric Cancer (GC). The aim of this study was to consider the expression of NEAT1, lincRNA-ROR and Oct4 and finally evaluate the correlation between their expression and clinical characteristics in Iranian patients with GC.

Methods: This cross-sectional study was performed on 41 gastric tumor tissue samples with matched normal adjacent tumor tissues. The RNA level of lncRNA NEAT1 and lincRNA ROR and Oct4 genes were assessed using the quantitative Real-time polymerase chain reaction. B2M was used as an internal control. Also, the relative expression of lncRNA NEAT1 and lincRNA ROR compared Oct4 in GC tissues was evaluated. The 2-ΔΔCq method was used to determine the expression fold changes.

Results: A significant association was observed between the levels of lincRNA ROR and Oct4 genes in gastric tumor tissues comparing normal adjacent tissues (Mean=1.558, p=0.014), (Mean=3.337, p<0.001) and (Mean=4.385, p<0.001), respectively. In addition, clinicopathological data comparing with lncRNAs and Oct4 mRNA expression levels in gastric cancer tissues showed no significant association. Here, we found that significant association between the levels of lincRNA ROR expression comparing oct4 mRNA level in gastric cancer tissues (R=0.417; P=0.024). Conclusion: Our results showed that lincRNA ROR and Oct4 have a significant association in gastric cancer. Also, our results indicate the first suggestion that lincRNA ROR expression correlated with oct4 mRNA level in gastric cancer tissues.

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