Immunohistochemistry evaluation of CD40 in Iraqi patients with gastric cancer

Abstract

Author(s): Asraa Naduhm Abd-Alsatar* and Israa A. Dheeb

Background: CD40 is one of the most important cellular markers that involved in many immune reactions and pathological pathways. The present investigation purposes to examine the immune-histochemical expression of CD40 in Gastric Tumor (GC) patients in Iraq. Methods: Serum concentrations of CCL22 and CCL5 were determined in cases of GC after and before tumor eradication using ELISA kits, and the examination was carried out according to the manufacturer's instructions, while those indicators were determined in paraffin-embedded blocks of stomach tissues immunohistologically using the Immunohistochemistry (IHC) method. Results: Present study included 40 cases of gastric cancer. Age of the cases extended from 31 years to 78 years with an average about 55.3 years and most patients are males (60%). We found most of the patients in the fourth (70%) and third stages (25%), in which the cancer is considered to be in the advanced stage. IHC assay determine that CD40 are exceedingly articulated in gastric tumor cells. Serologically, CD40 was significantly higher (P<0.05) among cases before surgery (26.705 pg/ml) than after surgery (18.30 pg/ml) and controls (7.22 pg/ml). Current study found the level of CD40 evaluated in males compared to females but without statistical differences (P>0.05). Moreover, we found the highest concentration of CD40 after and before surgery in advanced metastatic cancer cases (stage IV). In Conclusion, we found that CD40 is histo-immunologically linked with GC, which may play a role in the progression and spread of GC. Therefore, CD40 can be relied upon in the diagnosis and treatment of GC.

Share this article