Roles of IL-6, TNF-α and β-catenin in patients with colorectal cancer

Abstract

Author(s): Raghad Abdullah Hassan, Suhad Saad Mahmood, Basem Ahmed Askar

Colorectal cancer (CRC) occurs when cells in the colon or rectum grow uncontrollably. In most cases, polyps form on the inner lining of the colon or rectum and progressively expand into a malignant polypy that causes colorectal cancer. The present work was aimed to investigate role some biomarkers, including IL-6, TNF-α and β-catenin in CRC disease. Fifty-six tissue specimens, including 31 colorectal tissue specimens and 25 healthy normal tissue specimens were collected from individuals, with different ages (ranged between 26 and 85 years) and sexes (males and females), were attained different hospitals in Baghdad city during the period of March 2021 and September 2022. The age of CRC patients was ranged between 38 and 85 years and the age of health controls was ranged between 26 and 85 years. 4 groups of age (50≤ years) and (50-59 years) and (60-69 years) and (≥69 years) were included. was significantly variation in the mean ± SD of age (60.68±12.69 vs. 51.12±14.53 year; p<0.01) among patients with CRC and healthy controls, respectively. Majority of individuals was males (64.3%; n=36), while females were 35.7% (n=20), whereas 61.3% (n=19) males and 38.7% (n=12) females diagnosed with CRC were involved. Also, 32.0% (n=8) women and 68.0% (n=17) men healthy controls were involved. According to results, these was no significantly variation between gender of CRC patients and healthy controls with p-value (<0.5). In order to study some biomarkers, including β-catenin, IL-6 and TNF-α, immunohistochemistry assay was utilized. There was a highly significantly variation in β-catenin, IL6 and TNF-α in tissue specimens between CRC cases and HCs with p-value <0.05, <0.001 and <0.001. In addition, the study indicated that these were significantly variations in β-catenin and both TNF-alpha and IL-6, in addition to IL-6 and TNF-alpha among CRC patients and controls.

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