The effects of different laser irradiation techniques on the proteome composition of human blood plasma with breast cancer

Abstract

Author(s): Ahmed Abdul Kareem AL-Kaabi, Mustafa S Al-Musawi, Ali Abdulatif Hasan

As a continuation of earlier laboratory research and its findings, we are studying the effects of biostimulation and alteration on human blood plasma to improve blood circulation in blood vessels, treat some infections, and treat various diseases, including blood protein-related ones. Blood samples with breast cancer were collected through venipuncture into tubes containing, ethylenediaminetetr acidic (EDTA) as an anticoagulant from healthy adult donors, and plasma was separated from blood components. Blood plasma samples were irradiated for varying periods of time (5,10,15, and 20) minutes. Before and after irradiation, total protein and albumin concentrations were calculated using 375 nm and 650 nm lasers. Using a spectrophotometer, the concentration of total protein and albumin was determined for each sample. At the (375 and 650) nm laser wavelength and exposure durations of (5,10,15, and20) minutes, it was observed that the total protein concentration had significant differences between pre- and postirradiation probate value (p-value) (0.05, 0.05, 0.05, and 0.05, respectively). It was observed that the total protein and albumin concentrations had significant differences between pre- and postirradiation. In addition, the results demonstrate that the concentration of total protein and albumin decreases more significantly at a laser wavelength of 650 nm as compared to a laser wavelength of 375 nm at times of (5 and 10) minutes. With preference given to the laser with a wavelength of 630 nm.

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