A Study on evaluation of chemokines and endoproteases in the human prostate cancer stem cells

Abstract

Author(s): Surampudi Sreedhar, Siva Kumar Kandula and Satyanarayana Swamy Cheekatla*

Prostate cancer, the greatest cause of mortality among men, is a prevalent disease. CSC’s role and paradigm in prostate cancer development and initiation has received a lot of attention in cancer biology since it provides insights into cancer pathophysiology and therapy responses. CSCs interact with their surroundings, altering cell fate by triggering key signaling regulators, culminating in a metastatic cascade. Chemokines and their receptors are involved in survival, proliferation, and invasion in primary and metastatic sites. According to recent data, these proteins work along with MMPs to improve tumour cell survival, proliferation, and metastasis in a multistep process. MMPs interact with host components to promote cancer growth. These could have a chemo-preventive effect on angiogenesis-related pathways such as stem cell markers and stem cell behaviour injunction. As a result, establishing a relationship between specific cell surface markers associated CSCs and MMPs aids in the development of prospective therapeutics and clinical trial-ready medications. Scant data is available to support the use of MMPs to prevent prostate cancer by counteracting CSC inactivation. The current study focused on the separation and characterization of PCa CSCs, in addition to the production of chemokines and MMPs. In isolated tertiary spheroidal Pca CSCs, there was prominent expression of CD133+ and CD44+ cell surface markers, as well as overexpression of OCT4 and ABCB1/MDR-1. CD133+44+sub population were expressing significantly high SDF-1α, CXCR-4 and endoprotreases on comparison with primary cultures of normal prostate and CD133+ subpopulation. PCa CSC double positive subpopulation has a better survival rate and may be involved metastatic angiogenic progression.

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