Evaluation Of The Efficiency Of Pyocyanin Purified From Pseudomonas Aeruginosa As Anticancer Agent Toward Human Breast Cancer Cell Line CAL-51

Abstract

Author(s): Mohammad J. Al-Jassani*, Manar Mohammed Hizam, Maha Noori Shakir, Sadeq Sahib Sadeq, Mutni A. majeed, Nathera Hussin Alwan and Zahraa F. Hassan

Pseudomonas aeruginosa was isolated from Tigris River. These
isolates could produce two types of pigments on the cetrimide agar
plates. The quantity of productivity for Pyocyanin pigment ranged
between 5.9 and 13.7 μg/ml and increased with increasing the
incubation period to 72 hours. The Pyocyanin was purified by silica gel
chromatography. The cytotoxic activity of the Pyocyanin on tumor cell
line CAL-51 and normal cell line REF using a range of Pyocyanin
concentrations caused a reduction in the cell viability in CAL-51 and
REF cell lines in a dose-dependent manner. The doses of 50, 100, 150
ng/ml, 200 ng/ml, and 400 ng/ml were non-significant in the inhibition
ratios of 14%, 20%, 24%, 27% and 37 %, respectively, in the REF cell
line, on the other hand, showed the best inhibition ratio with 33%,
45%, 52%, 64%, and 71%.

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