Mohammad J. Al-Jassani

Publications

• Research Article   
  Evaluation Of The Efficiency Of Pyocyanin Purified From Pseudomonas Aeruginosa As Anticancer Agent Toward Human Breast Cancer Cell Line CAL-51
  Author(s): Mohammad J. Al-Jassani*, Manar Mohammed Hizam, Maha Noori Shakir, Sadeq Sahib Sadeq, Mutni A. majeed, Nathera Hussin Alwan and Zahraa F. Hassan
  
  Pseudomonas aeruginosa was isolated from Tigris River. These isolates could produce two types of pigments on the cetrimide agar plates. The quantity of productivity for Pyocyanin pigment ranged between 5.9 and 13.7 μg/ml and increased with increasing the incubation period to 72 hours. The Pyocyanin was purified by silica gel chromatography. The cytotoxic activity of the Pyocyanin on tumor cell line CAL-51 and normal cell line REF using a range of Pyocyanin concentrations caused a reduction in the cell viability in CAL-51 and REF cell lines in a dose-dependent manner. The doses of 50, 100, 150 ng/ml, 200 ng/ml, and 400 ng/ml were non-significant in the inhibition ratios of 14%, 20%, 24%, 27% and 37 %, respectively, in the REF cell line, on the other hand, showed the best inhibition ratio with 33%, 45.. Read More»
  

  Abstract PDF